Difference between revisions of "Part:BBa K1642011:Experience"
Line 6: Line 6:
 
[[File:SJTU Pdark-HR D.png|400px|thumb|left|Figure1.Biodesalination process controlled by Pdark.]]Based on Pdark and HR, we constructed an improved biodesalination system which depends only on the switch between white light and darkness. In the growth stage and working stage, we provide white light, while in the induced expression stage the light source is removed. Darkness leads to starvation and the starvation can inhibit active export of sodium, which is essential for biodesalination in the working stage.(described l in section transport module). The biodesalination process controlled by Pdark is shown in Figure 1.
 
[[File:SJTU Pdark-HR D.png|400px|thumb|left|Figure1.Biodesalination process controlled by Pdark.]]Based on Pdark and HR, we constructed an improved biodesalination system which depends only on the switch between white light and darkness. In the growth stage and working stage, we provide white light, while in the induced expression stage the light source is removed. Darkness leads to starvation and the starvation can inhibit active export of sodium, which is essential for biodesalination in the working stage.(described l in section transport module). The biodesalination process controlled by Pdark is shown in Figure 1.
  
−
The effectiveness of this biodesalination process is proved by determination of the concentrations of extracelluar sodium and chloride or desalination assay, which are shown in Figure 4. During the early time of working stage, there is an obvious decrease of concention compared to that of wild-type, which proves the function of our biobrick(Park-HR, BBa_K1642011). An obvious decrease during the early time and a following rise are consistent with that of the process controlled by PcpcG2.
+
 
 +
 
 +
 
 +
 
 
[[File:SJTU Pdark-HR A1.png|360px|thumb|left|Figure2.Biodesalination assay of the process controlled by Pdark.]]
 
[[File:SJTU Pdark-HR A1.png|360px|thumb|left|Figure2.Biodesalination assay of the process controlled by Pdark.]]
 +
The effectiveness of this biodesalination process is proved by determination of the concentrations of extracelluar sodium and chloride or desalination assay, which are shown in Figure 4. During the early time of working stage, there is an obvious decrease of concention compared to that of wild-type, which proves the function of our biobrick(Park-HR, BBa_K1642011). An obvious decrease during the early time and a following rise are consistent with that of the process controlled by PcpcG2.
  
 +
 +
 +
 +
[[File:SJTU Pdark-HR A2.png|360px|thumb|left|Figure3. Biodesalination assay of the process controlled by Pdark with more frequent sampling times.]]
 
To figure out the limitation of desalination, we prolonged the length of the induction stage and adjusted the times of taking samples. The results are shown in Figure 5. The 6h in the working stage is approximately the minimum point.
 
To figure out the limitation of desalination, we prolonged the length of the induction stage and adjusted the times of taking samples. The results are shown in Figure 5. The 6h in the working stage is approximately the minimum point.
−
<center>{{ Template:SJTU-BioX-Shanghai/Figure
 
−
| figure = [[File:SJTUB_biode(61).jpg| 410px | frameless ]]
 
−
| float = middle
 
−
| id = 1.4.6
 
−
| label = Biodesalination assay of the process controlled by Pdark with more frequent sampling times.
 
−
| descr = The start of induction stage is at -16h and the times of taking samples are at -16h, 0h, 2h, 2h, 4h, 6h, 8h,10h,12h.
 
−
}}</center>
 
  
 
The acquisition of the minimum point makes it possible to design a longer biodesalination process. We can extend this process by alternating induction stage (starvation stage) and working stage, make the cyanobacteria to experience starvation and regain of energy for more cycles, thus achieving more reduction of salinity. Moreover, if the length of the induction stage and working stage are approximately 12h, after growth stage this improved biodesalination system can be controlled by the natural alternation between day and night without any human intervention.
 
The acquisition of the minimum point makes it possible to design a longer biodesalination process. We can extend this process by alternating induction stage (starvation stage) and working stage, make the cyanobacteria to experience starvation and regain of energy for more cycles, thus achieving more reduction of salinity. Moreover, if the length of the induction stage and working stage are approximately 12h, after growth stage this improved biodesalination system can be controlled by the natural alternation between day and night without any human intervention.
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
  
 
===User Reviews===
 
===User Reviews===

Revision as of 22:22, 18 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1642011

Figure1.Biodesalination process controlled by Pdark.
Based on Pdark and HR, we constructed an improved biodesalination system which depends only on the switch between white light and darkness. In the growth stage and working stage, we provide white light, while in the induced expression stage the light source is removed. Darkness leads to starvation and the starvation can inhibit active export of sodium, which is essential for biodesalination in the working stage.(described l in section transport module). The biodesalination process controlled by Pdark is shown in Figure 1.



Figure2.Biodesalination assay of the process controlled by Pdark.

The effectiveness of this biodesalination process is proved by determination of the concentrations of extracelluar sodium and chloride or desalination assay, which are shown in Figure 4. During the early time of working stage, there is an obvious decrease of concention compared to that of wild-type, which proves the function of our biobrick(Park-HR, BBa_K1642011). An obvious decrease during the early time and a following rise are consistent with that of the process controlled by PcpcG2.



Figure3. Biodesalination assay of the process controlled by Pdark with more frequent sampling times.

To figure out the limitation of desalination, we prolonged the length of the induction stage and adjusted the times of taking samples. The results are shown in Figure 5. The 6h in the working stage is approximately the minimum point.

The acquisition of the minimum point makes it possible to design a longer biodesalination process. We can extend this process by alternating induction stage (starvation stage) and working stage, make the cyanobacteria to experience starvation and regain of energy for more cycles, thus achieving more reduction of salinity. Moreover, if the length of the induction stage and working stage are approximately 12h, after growth stage this improved biodesalination system can be controlled by the natural alternation between day and night without any human intervention.








User Reviews

UNIQ46cd1b7a12197a19-partinfo-00000000-QINU UNIQ46cd1b7a12197a19-partinfo-00000001-QINU