Difference between revisions of "Part:BBa K1796106"
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<partinfo>BBa_K1796106 short</partinfo> | <partinfo>BBa_K1796106 short</partinfo> | ||
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<partinfo>BBa_K1796106 parameters</partinfo> | <partinfo>BBa_K1796106 parameters</partinfo> | ||
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| + | ==Parameter of Protein== | ||
| + | <p> Number of amino acids: 306 </p> | ||
| + | <p> </p> | ||
| + | <p> Molecular weight: 33924.5 </p> | ||
| + | <p> </p> | ||
| + | <p> Theoretical pI: 8.27 </p> | ||
| + | <p> </p> | ||
| + | <p> Amino acid composition: </p> | ||
| + | <p> </p> | ||
| + | <p> Ala (A) 32 10.5% </p> | ||
| + | <p> </p> | ||
| + | <p> Arg (R) 18 5.9% </p> | ||
| + | <p> </p> | ||
| + | <p> Asn (N) 9 2.9% </p> | ||
| + | <p> </p> | ||
| + | <p> Asp (D) 17 5.6% </p> | ||
| + | <p> </p> | ||
| + | <p> Cys (C) 4 1.3% </p> | ||
| + | <p> </p> | ||
| + | <p> Gln (Q) 15 4.9% </p> | ||
| + | <p> </p> | ||
| + | <p> Glu (E) 21 6.9% </p> | ||
| + | <p> </p> | ||
| + | <p> Gly (G) 21 6.9% </p> | ||
| + | <p> </p> | ||
| + | <p> His (H) 9 2.9% </p> | ||
| + | <p> </p> | ||
| + | <p> Ile (I) 26 8.5% </p> | ||
| + | <p> </p> | ||
| + | <p> Leu (L) 22 7.2% </p> | ||
| + | <p> </p> | ||
| + | <p> Lys (K) 22 7.2% </p> | ||
| + | <p> </p> | ||
| + | <p> Met (M) 14 4.6% </p> | ||
| + | <p> </p> | ||
| + | <p> Phe (F) 12 3.9% </p> | ||
| + | <p> </p> | ||
| + | <p> Pro (P) 14 4.6% </p> | ||
| + | <p> </p> | ||
| + | <p> Ser (S) 14 4.6% </p> | ||
| + | <p> </p> | ||
| + | <p> Thr (T) 7 2.3% </p> | ||
| + | <p> </p> | ||
| + | <p> Trp (W) 1 0.3% </p> | ||
| + | <p> </p> | ||
| + | <p> Tyr (Y) 4 1.3% </p> | ||
| + | <p> </p> | ||
| + | <p> Val (V) 24 7.8% </p> | ||
| + | <p> </p> | ||
| + | <p> Pyl (O) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> Sec (U) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> (B) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> (Z) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> (X) 0 0.0% </p> | ||
| + | <p> </p> | ||
| + | <p> Total number of negatively charged residues (Asp + Glu): 38 </p> | ||
| + | <p> Total number of positively charged residues (Arg + Lys): 40 </p> | ||
| + | <p> </p> | ||
| + | <p> Atomic composition:Carbon C 1501 </p> | ||
| + | <p> Hydrogen H 2435 </p> | ||
| + | <p> Nitrogen N 425 </p> | ||
| + | <p> Oxygen O 432 </p> | ||
| + | <p> Sulfur S 18 </p> | ||
| + | <p> </p> | ||
| + | <p> Formula: C1501H2435N425O432S18Total number of atoms: 4811 </p> | ||
| + | <p> </p> | ||
| + | <p> Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. </p> | ||
| + | <p> </p> | ||
| + | <p> Ext. coefficient 11710 </p> | ||
| + | <p> Abs 0.1% (=1 g/l) 0.345, assuming all pairs of Cys residues form cystines </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> Ext. coefficient 11460 </p> | ||
| + | <p> Abs 0.1% (=1 g/l) 0.338, assuming all Cys residues are reduced </p> | ||
| + | <p> </p> | ||
| + | <p> Estimated half-life:The N-terminal of the sequence considered is M (Met). </p> | ||
| + | <p> </p> | ||
| + | <p> The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). </p> | ||
| + | <p> >20 hours (yeast, in vivo). </p> | ||
| + | <p> >10 hours (Escherichia coli, in vivo). </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> Instability index:The instability index (II) is computed to be 36.95 </p> | ||
| + | <p> This classifies the protein as stable. </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> </p> | ||
| + | <p> Aliphatic index: 94.38 </p> | ||
| + | <p> </p> | ||
| + | <p> Grand average of hydropathicity (GRAVY): -0.119 </p> | ||
Latest revision as of 22:20, 18 September 2015
methylene-H4folate reductase
This reductase has catalysis of turning methylene-H4folate into methyl-H4folate, then the methyl can be taken by CFeSP.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 808
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 808
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 808
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 808
Illegal NgoMIV site found at 544
Illegal NgoMIV site found at 652
Illegal NgoMIV site found at 829
Illegal NgoMIV site found at 889 - 1000COMPATIBLE WITH RFC[1000]
Parameter of Protein
Number of amino acids: 306
Molecular weight: 33924.5
Theoretical pI: 8.27
Amino acid composition:
Ala (A) 32 10.5%
Arg (R) 18 5.9%
Asn (N) 9 2.9%
Asp (D) 17 5.6%
Cys (C) 4 1.3%
Gln (Q) 15 4.9%
Glu (E) 21 6.9%
Gly (G) 21 6.9%
His (H) 9 2.9%
Ile (I) 26 8.5%
Leu (L) 22 7.2%
Lys (K) 22 7.2%
Met (M) 14 4.6%
Phe (F) 12 3.9%
Pro (P) 14 4.6%
Ser (S) 14 4.6%
Thr (T) 7 2.3%
Trp (W) 1 0.3%
Tyr (Y) 4 1.3%
Val (V) 24 7.8%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 40
Atomic composition:Carbon C 1501
Hydrogen H 2435
Nitrogen N 425
Oxygen O 432
Sulfur S 18
Formula: C1501H2435N425O432S18Total number of atoms: 4811
Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 11710
Abs 0.1% (=1 g/l) 0.345, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.338, assuming all Cys residues are reduced
Estimated half-life:The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:The instability index (II) is computed to be 36.95
This classifies the protein as stable.
Aliphatic index: 94.38
Grand average of hydropathicity (GRAVY): -0.119