Difference between revisions of "Part:BBa K1627000"

 
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<partinfo>BBa_K1627000 short</partinfo>
 
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This part contains genes that should allow for the demethylation of 1-methylxanthine. The part contains a synthetic operon consisting of ndmA, ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.  
 
This part contains genes that should allow for the demethylation of 1-methylxanthine. The part contains a synthetic operon consisting of ndmA, ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.  
  
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While this device should be able to demethylate the 1-methyl group on methylxanthines, the ndmA gene lacks specificity to 1-methylxanthine. Thus, this part is not actually able to be used as designed. However, this part was important for the construction of parts BBa_K1627003, BBa_K1627004, and BBa_K1627006, which are able to demethylate the 1-methylgroup from theophylline, paraxanthine, and caffeine, respectively.
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While this device should be able to demethylate the 1-methyl group on methylxanthines, the ndmA gene lacks specificity to 1-methylxanthine. Thus, this part is not actually able to be used as designed. However, this part was important for the construction of parts BBa_K1627003, BBa_K1627004, and BBa_K1627006, which are able to demethylate the 1-methyl group from theophylline, paraxanthine, and caffeine, respectively.
  
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===Usage and Biology===
 
===Usage and Biology===
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[[Image:2015_Austin_UTexas_PDCAFA_growth_curve.png]]
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Revision as of 22:18, 18 September 2015

Device to demethylate 1-methylxanthine

This part contains genes that should allow for the demethylation of 1-methylxanthine. The part contains a synthetic operon consisting of ndmA, ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.

While this device should be able to demethylate the 1-methyl group on methylxanthines, the ndmA gene lacks specificity to 1-methylxanthine. Thus, this part is not actually able to be used as designed. However, this part was important for the construction of parts BBa_K1627003, BBa_K1627004, and BBa_K1627006, which are able to demethylate the 1-methyl group from theophylline, paraxanthine, and caffeine, respectively.

Usage and Biology

2015 Austin UTexas PDCAFA growth curve.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1199
    Illegal BglII site found at 1670
    Illegal XhoI site found at 2811
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3509
    Illegal AgeI site found at 1338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 983