Difference between revisions of "Part:BBa K1796105"

 
 
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<partinfo>BBa_K1796105 short</partinfo>
 
<partinfo>BBa_K1796105 short</partinfo>
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<partinfo>BBa_K1796105 parameters</partinfo>
 
<partinfo>BBa_K1796105 parameters</partinfo>
 
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==Parameter of Protein==
 +
<p> Number of amino acids: 446 </p>
 +
<p> </p>
 +
<p> Molecular weight: 48153.2 </p>
 +
<p> </p>
 +
<p> Theoretical pI: 5.38 </p>
 +
<p> </p>
 +
<p> Amino acid composition:  </p>
 +
<p> </p>
 +
<p> Ala (A)  65      14.6% </p>
 +
<p> </p>
 +
<p> Arg (R)  19      4.3% </p>
 +
<p> </p>
 +
<p> Asn (N)  19      4.3% </p>
 +
<p> </p>
 +
<p> Asp (D)  26      5.8% </p>
 +
<p> </p>
 +
<p> Cys (C)  5      1.1% </p>
 +
<p> </p>
 +
<p> Gln (Q)  10      2.2% </p>
 +
<p> </p>
 +
<p> Glu (E)  31      7.0% </p>
 +
<p> </p>
 +
<p> Gly (G)  25      5.6% </p>
 +
<p> </p>
 +
<p> His (H)  7      1.6% </p>
 +
<p> </p>
 +
<p> Ile (I)  24      5.4% </p>
 +
<p> </p>
 +
<p> Leu (L)  49      11.0% </p>
 +
<p> </p>
 +
<p> Lys (K)  28      6.3% </p>
 +
<p> </p>
 +
<p> Met (M)  6      1.3% </p>
 +
<p> </p>
 +
<p> Phe (F)  11      2.5% </p>
 +
<p> </p>
 +
<p> Pro (P)  21      4.7% </p>
 +
<p> </p>
 +
<p> Ser (S)  21      4.7% </p>
 +
<p> </p>
 +
<p> Thr (T)  24      5.4% </p>
 +
<p> </p>
 +
<p> Trp (W)  2      0.4% </p>
 +
<p> </p>
 +
<p> Tyr (Y)  16      3.6% </p>
 +
<p> </p>
 +
<p> Val (V)  37      8.3% </p>
 +
<p> </p>
 +
<p> Pyl (O)  0      0.0% </p>
 +
<p> </p>
 +
<p> Sec (U)  0      0.0% </p>
 +
<p> </p>
 +
<p> (B)  0          0.0% </p>
 +
<p> </p>
 +
<p> (Z)  0          0.0% </p>
 +
<p> </p>
 +
<p> (X)  0          0.0% </p>
 +
<p> </p>
 +
<p> Total number of negatively charged residues (Asp + Glu): 57 </p>
 +
<p> Total number of positively charged residues (Arg + Lys): 47 </p>
 +
<p> </p>
 +
<p> Atomic composition:Carbon      C              2151 </p>
 +
<p> Hydrogen    H        3454 </p>
 +
<p> Nitrogen    N          576 </p>
 +
<p> Oxygen      O          651 </p>
 +
<p> Sulfur      S          11 </p>
 +
<p> </p>
 +
<p> Formula: C2151H3454N576O651S11Total number of atoms: 6843 </p>
 +
<p> </p>
 +
<p> Extinction coefficients:Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water. </p>
 +
<p> </p>
 +
<p> Ext. coefficient    35090 </p>
 +
<p> Abs 0.1% (=1 g/l)  0.729, assuming all pairs of Cys residues form cystines </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Ext. coefficient    34840 </p>
 +
<p> Abs 0.1% (=1 g/l)  0.724, assuming all Cys residues are reduced </p>
 +
<p> </p>
 +
<p> Estimated half-life:The N-terminal of the sequence considered is M (Met). </p>
 +
<p> </p>
 +
<p> The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). </p>
 +
<p>                             >20 hours (yeast, in vivo). </p>
 +
<p>                             >10 hours (Escherichia coli, in vivo). </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Instability index:The instability index (II) is computed to be 25.50 </p>
 +
<p> This classifies the protein as stable. </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Aliphatic index: 102.47 </p>
 +
<p> </p>
 +
<p> Grand average of hydropathicity (GRAVY): 0.008 </p>

Latest revision as of 22:14, 18 September 2015

ACS chaperone

This sequence code a portion, which is helping ACS to fold in right way. If you need to product ACS and want it to have functions, this protein is necessary.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 299
    Illegal NgoMIV site found at 403
    Illegal NgoMIV site found at 422
    Illegal NgoMIV site found at 493
    Illegal NgoMIV site found at 690
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 446

Molecular weight: 48153.2

Theoretical pI: 5.38

Amino acid composition: 

Ala (A) 65 14.6%

Arg (R) 19 4.3%

Asn (N) 19 4.3%

Asp (D) 26 5.8%

Cys (C) 5 1.1%

Gln (Q) 10 2.2%

Glu (E) 31 7.0%

Gly (G) 25 5.6%

His (H) 7 1.6%

Ile (I) 24 5.4%

Leu (L) 49 11.0%

Lys (K) 28 6.3%

Met (M) 6 1.3%

Phe (F) 11 2.5%

Pro (P) 21 4.7%

Ser (S) 21 4.7%

Thr (T) 24 5.4%

Trp (W) 2 0.4%

Tyr (Y) 16 3.6%

Val (V) 37 8.3%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 57

Total number of positively charged residues (Arg + Lys): 47

Atomic composition:Carbon C 2151

Hydrogen H 3454

Nitrogen N 576

Oxygen O 651

Sulfur S 11

Formula: C2151H3454N576O651S11Total number of atoms: 6843

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 35090

Abs 0.1% (=1 g/l) 0.729, assuming all pairs of Cys residues form cystines

Ext. coefficient 34840

Abs 0.1% (=1 g/l) 0.724, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 25.50

This classifies the protein as stable.

Aliphatic index: 102.47

Grand average of hydropathicity (GRAVY): 0.008