Difference between revisions of "Part:BBa K1602017"
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         <h1><small>D</small>-xylonic acid producing operon</h1>
 
         <h1><small>D</small>-xylonic acid producing operon</h1>
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         <small>D</small>-Xylose is a monosaccharide belonging to the aldopentose family. It was recently    shown that the <small>D</small>-xylose dehydrogenase <i>xylB</i> from <i>Caulobacter crescentus</i> can convert <small>D</small>-xylose to <small>D</small>-xylonolactone. This can react spontaneously or through the catalysation of <i>xylC</i> to <small>D</small>-xylonic acid. In <i>E. coli</i> <small>D</small>-xylonic acid can be further metabolized to ethyleneglycol.(1)
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         <small>D</small>-Xylose is a monosaccharide belonging to the aldopentose family. It was recently    shown that the <small>D</small>-xylose dehydrogenase <i>xylB</i> from <i>Caulobacter crescentus</i> can convert <small>D</small>-xylose to <small>D</small>-xylonolactone. This can react spontaneously or through the catalysation of <i>xylC</i> to <small>D</small>-xylonic acid.  
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In <i>E. coli</i> <small>D</small>-xylonic acid can be further metabolized to ethyleneglycol by the enzymes yjhG <a href="https://parts.igem.org/Part:BBa_K1602012">BBa_K1602012</a> yagE <a href="https://parts.igem.org/Part:BBa_K1602011">BBa_K1602011</a> and yqhD <a href="https://parts.igem.org/Part:BBa_K1602013">BBa_K1602013</a> which are already present in this host.(1)
 
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Revision as of 22:13, 18 September 2015

D-xylonic acid producing operon

D-Xylose is a monosaccharide belonging to the aldopentose family. It was recently shown that the D-xylose dehydrogenase xylB from Caulobacter crescentus can convert D-xylose to D-xylonolactone. This can react spontaneously or through the catalysation of xylC to D-xylonic acid.

In E. coli D-xylonic acid can be further metabolized to ethyleneglycol by the enzymes yjhG BBa_K1602012 yagE BBa_K1602011 and yqhD BBa_K1602013 which are already present in this host.(1)


https://static.igem.org/mediawiki/parts/f/f1/TU_Darmstadt_EG_XylB-xylC.png

  • Figure 1 Sheme of the reactions catalyzed by xylB and xylC. The xylC reaction can also happen spontaneously but in a much lower speed.(2)



    • Usage

    This part is a composite of two coding genes with strong RBS (BBa_B0034). The transcription is controlled by a T7 promotor (BBa_I719005).

    We used this operon to investigate possible production of ethylene glycol in E. coli.


    Figure 2


    Results
    Figure 3 Scan of the PAGE containing from left to right a marker (M; Protein Marker III AppliChem), the positive sample (1) and a negative control (2). The picture was cropped and edited for clarification purposes.
    Figure 4 Plot of the gel lanes based on contrast analyses - created with ImageJ

    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      COMPATIBLE WITH RFC[25]
    • 1000
      COMPATIBLE WITH RFC[1000]


    References

    1. Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Lee WK, Chung WJ. Biosynthesis of ethylene glycol in Escherichia coli. Appl Microbiol Biotechnol. 2013;97(8):3409-17.

    2. Toivari MH, Nygard Y, Penttila M, Ruohonen L, Wiebe MG. Microbial D-xylonate production. Appl Microbiol Biotechnol. 2012;96(1):1-8.