Difference between revisions of "Part:BBa E0422"
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'''Figure 1: Fluorescence microscopy of ''E. coli'' DH5alpha bearing K170728''' | '''Figure 1: Fluorescence microscopy of ''E. coli'' DH5alpha bearing K170728''' | ||
| − | [[File:Paris_Saclay_ K1707028_w1POL.png| | + | [[File:Paris_Saclay_ K1707028_w1POL.png|200px]] |
Differential interference contrast | Differential interference contrast | ||
| − | [[File:Paris_Saclay_K1707028_w2Lumencor CFP 434-17.png| | + | [[File:Paris_Saclay_K1707028_w2Lumencor CFP 434-17.png|200px]] |
Excitation with led 437 nm/17nm (CFP), 100 ms of exposure | Excitation with led 437 nm/17nm (CFP), 100 ms of exposure | ||
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Revision as of 22:12, 18 September 2015
ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)
Cyan fluorescent protein with ribosome binding site and terminator
Usage and Biology
This part can be used to test promoter strength or the proper functioning of a regulator device. The LVA tail is particularly useful when testing a regulatory device, for example to assess the efficiency of repressor.
In this experiment, the E0422 part was associated with a cI regulated promoter (part R0051), resulting in K1707028.
Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing K170728
Differential interference contrast
Excitation with led 437 nm/17nm (CFP), 100 ms of exposure
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]