Difference between revisions of "Part:BBa E0422"

Revision as of 22:11, 18 September 2015

ECFP (RBS+ LVA+ TERM) (B0034.E0022.B0015)

Cyan fluorescent protein with ribosome binding site and terminator

Usage and Biology

This part can be used to test promoter strength or the proper functioning of a regulator device. The LVA tail is particularly useful when testing a regulatory device, for example to assess the efficiency of repressor.

In this experiment, the E0422 part was associated with a cI regulated promoter (part R0051), resulting in K1707028.

Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing K170728

Differential interference contrast

Excitation with led 437 nm/17nm (CFP), 100 ms of exposure Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_E0422 short</partinfo>
--- No description --
+Cyan fluorescent protein with ribosome binding site and terminator
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part can be used to test promoter strength or the proper functioning of a regulator device.  The LVA tail is particularly useful when testing a regulatory device, for example to assess the efficiency of repressor.
+In this experiment, the E0422 part was associated with a cI regulated promoter (part R0051), resulting in K1707028.
+'''Figure 1: Fluorescence microscopy of ''E. coli'' DH5alpha bearing K170728'''
+[[File:Paris_Saclay_ K1707028_w1POL.png|300px]]
+Differential interference contrast
+[[File:Paris_Saclay_K1707028_w2Lumencor CFP 434-17.png|300px]]
+Excitation with led 437 nm/17nm (CFP), 100 ms of exposure
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>