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Revision as of 22:08, 18 September 2015
Escherichia coli Polyphosphate Kinase Enzyme
This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from Escherichia coli. PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the γ-phosphate of ATP to the end of the polyphosphate chain (Akiyama et al., 1992). This enzyme is responsible for the formation of long chain polyphosphate molecules (up to a thousand orthophosphate residues long) - see illustration below.
![](https://static.igem.org/mediawiki/2015/c/c7/Pst_Activity_York.jpeg)
Akiyama, Masahiro, E. Crooke, and Arthur Kornberg. "The polyphosphate kinase gene of Escherichia coli. Isolation and sequence of the ppk gene and membrane location of the protein." Journal of Biological Chemistry 267.31 (1992): 22556-22561.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 396
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1750
- 1000COMPATIBLE WITH RFC[1000]