Difference between revisions of "Part:BBa K1796007"
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==Parametre of Protein== | ==Parametre of Protein== | ||
− | + | Number of amino acids: 499 | |
− | + | ||
− | + | Molecular weight: 54869.8 | |
− | + | ||
− | + | Theoretical pI: 6.64 | |
− | + | ||
− | + | Amino acid composition: | |
− | + | Ala (A) 46 9.2% | |
− | + | Arg (R) 35 7.0% | |
− | + | Asn (N) 17 3.4% | |
− | + | Asp (D) 23 4.6% | |
− | + | Cys (C) 16 3.2% | |
− | + | Gln (Q) 19 3.8% | |
− | + | Glu (E) 40 8.0% | |
− | + | Gly (G) 42 8.4% | |
− | + | His (H) 17 3.4% | |
− | + | Ile (I) 30 6.0% | |
− | + | Leu (L) 41 8.2% | |
− | + | Lys (K) 25 5.0% | |
− | + | Met (M) 11 2.2% | |
− | + | Phe (F) 13 2.6% | |
− | + | Pro (P) 25 5.0% | |
− | + | Ser (S) 27 5.4% | |
− | + | Thr (T) 17 3.4% | |
− | + | Trp (W) 2 0.4% | |
− | + | Tyr (Y) 13 2.6% | |
− | + | Val (V) 40 8.0% | |
− | + | Pyl (O) 0 0.0% | |
− | + | Sec (U) 0 0.0% | |
− | + | ||
− | + | (B) 0 0.0% | |
− | + | (Z) 0 0.0% | |
− | + | (X) 0 0.0% | |
− | + | ||
− | + | ||
− | + | Total number of negatively charged residues (Asp + Glu): 63 | |
− | + | Total number of positively charged residues (Arg + Lys): 60 | |
− | + | ||
− | + | Atomic composition: | |
− | + | ||
− | + | Carbon C 2398 | |
− | + | Hydrogen H 3849 | |
− | + | Nitrogen N 701 | |
− | + | Oxygen O 719 | |
− | + | Sulfur S 27 | |
− | + | ||
− | + | Formula: C2398H3849N701O719S27 | |
− | + | Total number of atoms: 7694 | |
− | + | ||
− | + | Extinction coefficients: | |
− | + | ||
− | + | Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. | |
− | + | ||
− | + | Ext. coefficient 31370 | |
− | + | Abs 0.1% (=1 g/l) 0.572, assuming all pairs of Cys residues form cystines | |
− | + | ||
− | + | ||
− | + | Ext. coefficient 30370 | |
− | + | Abs 0.1% (=1 g/l) 0.553, assuming all Cys residues are reduced | |
− | + | ||
− | + | Estimated half-life: | |
− | + | ||
− | + | The N-terminal of the sequence considered is M (Met). | |
− | + | ||
− | + | The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). | |
− | + | >20 hours (yeast, in vivo). | |
− | + | >10 hours (Escherichia coli, in vivo). | |
− | + | ||
− | + | ||
− | + | Instability index: | |
− | + | ||
− | + | The instability index (II) is computed to be 43.03 | |
− | + | This classifies the protein as unstable. | |
− | + | ||
− | + | ||
− | + | ||
− | + | Aliphatic index: 87.96 | |
− | + | ||
− | + | Grand average of hydropathicity (GRAVY): -0.253 | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
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Revision as of 22:02, 18 September 2015
nifB from Paenibacillus sp. WLY78
essential for biosynthesis of the active-site nitrogenase cofactor and encodes a radical A-adenosylmethionine(SAM)-dependent enzyme that inserts the central carbon atom into the eight-Fe core of nifB cofactor(nifB-co).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1156
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1156
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1156
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1156
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1156
Illegal AgeI site found at 129
Illegal AgeI site found at 1089
Illegal AgeI site found at 1444 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1261
Parametre of Protein
Number of amino acids: 499
Molecular weight: 54869.8
Theoretical pI: 6.64
Amino acid composition: Ala (A) 46 9.2% Arg (R) 35 7.0% Asn (N) 17 3.4% Asp (D) 23 4.6% Cys (C) 16 3.2% Gln (Q) 19 3.8% Glu (E) 40 8.0% Gly (G) 42 8.4% His (H) 17 3.4% Ile (I) 30 6.0% Leu (L) 41 8.2% Lys (K) 25 5.0% Met (M) 11 2.2% Phe (F) 13 2.6% Pro (P) 25 5.0% Ser (S) 27 5.4% Thr (T) 17 3.4% Trp (W) 2 0.4% Tyr (Y) 13 2.6% Val (V) 40 8.0% Pyl (O) 0 0.0% Sec (U) 0 0.0%
(B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 60
Atomic composition:
Carbon C 2398 Hydrogen H 3849 Nitrogen N 701 Oxygen O 719 Sulfur S 27
Formula: C2398H3849N701O719S27 Total number of atoms: 7694
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 31370 Abs 0.1% (=1 g/l) 0.572, assuming all pairs of Cys residues form cystines
Ext. coefficient 30370
Abs 0.1% (=1 g/l) 0.553, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.03 This classifies the protein as unstable.
Aliphatic index: 87.96
Grand average of hydropathicity (GRAVY): -0.253