Difference between revisions of "Part:BBa K1688015"
m |
|||
Line 2: | Line 2: | ||
<partinfo>BBa_K1688015 short</partinfo> | <partinfo>BBa_K1688015 short</partinfo> | ||
− | Upper naphthalene breakdown pathway from the Nah7 plasmid of ''Pseudomonas putida'' + RBS B0034. | + | Upper naphthalene breakdown pathway from the Nah7 plasmid of ''Pseudomonas putida'' + RBS B0034. The operon consists of ten genes (NahAa, NahAb, NahAc, NahAd, NahB, NahC, NahD, NahE, NahF, NahQ) which in turn codes for seven enzymes that catalyse the breakdown of naphthalene into salicylate. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 22:00, 18 September 2015
Nah7 - Upper naphthalene breakdown pathway (inc RBS)
Upper naphthalene breakdown pathway from the Nah7 plasmid of Pseudomonas putida + RBS B0034. The operon consists of ten genes (NahAa, NahAb, NahAc, NahAd, NahB, NahC, NahD, NahE, NahF, NahQ) which in turn codes for seven enzymes that catalyse the breakdown of naphthalene into salicylate.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1884
Illegal PstI site found at 5879 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 4139
Illegal PstI site found at 1884
Illegal PstI site found at 5879
Illegal NotI site found at 8182 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 5232
Illegal BamHI site found at 3217
Illegal BamHI site found at 5919
Illegal XhoI site found at 1328
Illegal XhoI site found at 3716
Illegal XhoI site found at 3868
Illegal XhoI site found at 9370 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1884
Illegal PstI site found at 5879 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1884
Illegal PstI site found at 5879
Illegal NgoMIV site found at 1821
Illegal NgoMIV site found at 3596
Illegal NgoMIV site found at 5470
Illegal NgoMIV site found at 6082
Illegal NgoMIV site found at 9395
Illegal AgeI site found at 659
Illegal AgeI site found at 3590
Illegal AgeI site found at 6298
Illegal AgeI site found at 8293 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2938
Illegal BsaI site found at 3611
Illegal BsaI site found at 4202
Illegal BsaI site found at 5368
Illegal BsaI.rc site found at 2772
Illegal BsaI.rc site found at 5522
Illegal BsaI.rc site found at 7807
Illegal SapI.rc site found at 3302
Illegal SapI.rc site found at 3848
Illegal SapI.rc site found at 7699