Difference between revisions of "Part:BBa K1813007"
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<h2>Background of <i>nicD</i>:</h2>
 
<h2>Background of <i>nicD</i>:</h2>
 
<h4>Part Description:</h4>
 
<h4>Part Description:</h4>
−
<p>Codon optimized coding sequence of <i>nicD</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813021">BBa_K1813021</a>. </p>
+
<p>Codon optimized coding sequence of <i>nicD</i>, an enzyme from the <i>Pseudmonas putida</i> bacterium KT2240, for E.Coli. This composite part was used to make our part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1813015">BBa_K1813015</a> </html> and
 +
<html><a href="https://parts.igem.org/Part:BBa_K1813021">BBa_K1813021</a> </html>.
 +
 
  
 
<h4>Part Origin:</h4>
 
<h4>Part Origin:</h4>
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<h4>Part Function:</h4>
 
<h4>Part Function:</h4>
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<p>The enzyme coded for by <i>nicD</i> converts N-FormylMaleamic (NFM) to Maleamic Acid.</p>
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<p>The enzyme coded for by <i>nicD</i> is a N-Formylmaleamate Deformylase that converts N-Formylmaleamic (NFM) to Maleamic Acid, also producing formic acid.</p>
  
 
<h4>References:</h4>
 
<h4>References:</h4>
 
[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105
 
[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105

Revision as of 21:58, 18 September 2015

nicD

nicD

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 727
  • 1000
    COMPATIBLE WITH RFC[1000]


Background of nicD:

Part Description:

Codon optimized coding sequence of nicD, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813015 and BBa_K1813021 .


Part Origin:

Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].

Part Function:

The enzyme coded for by nicD is a N-Formylmaleamate Deformylase that converts N-Formylmaleamic (NFM) to Maleamic Acid, also producing formic acid.

References:

[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105