Difference between revisions of "Part:BBa K1813009"
Line 2: Line 2:
 
<partinfo>BBa_K1813009 short</partinfo>
 
<partinfo>BBa_K1813009 short</partinfo>
  
−
nicF
+
<i>nicF</i> Gen Bank accession number Q88FY5.1.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:57, 18 September 2015

nicF

nicF Gen Bank accession number Q88FY5.1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 439
  • 1000
    COMPATIBLE WITH RFC[1000]


Background of nicF:

Part Description:

Codon optimized coding sequence of nicF, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813023.

Part Origin:

Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].

Part Function:

The enzyme coded for by nicF converts Maleamic Acid to Maleic Acid.

References:

[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105