Difference between revisions of "Part:BBa K1850012:Design"

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===Design Notes===
 
===Design Notes===
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We selected <partinfo>BBa_I13453</partinfo>, an arabinose promoter, because it is a titratable promoter that allows for controlled expression of pili structural genes over a broad range of inducer concentrations.
 
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''fim'' was amplified with the native ribosomal binding sites, so that we could maintain the dosing of the ''fim'' subunits.
 
''fim'' was amplified with the native ribosomal binding sites, so that we could maintain the dosing of the ''fim'' subunits.
  

Latest revision as of 21:56, 18 September 2015

fim


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2585
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 868
    Illegal AgeI site found at 899
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

fim was amplified with the native ribosomal binding sites, so that we could maintain the dosing of the fim subunits.

Source

The type I pili structural and transport genes from the fim operon were amplified from the E. coli K-12 genome.

References