Difference between revisions of "Part:BBa K1799021"

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<partinfo>BBa_K1799021 short</partinfo>
 
<partinfo>BBa_K1799021 short</partinfo>
  
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Device was designed and assembled using Synbiota's GENtle software and Rapid DNA Prototyping (RDP) system.  Part consists of a Lac promoter, medium RBS, and RFP open reading frame.  In LacI- strains, or when cultured in the presence of IPTG, RFP expression is up-regulated. We created this device to use as a control in our experiments testing other devices.
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Device was designed and assembled using Synbiota's Rapid DNA Prototyping (RDP) system.  Part consists of a Lac promoter, medium RBS, and RFP open reading frame.  In LacI- strains, or when cultured in the presence of IPTG, RFP expression is up-regulated. We created this device to use as a control in our experiments testing other devices.
  
 
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Revision as of 21:55, 18 September 2015

IPTG-inducible generator of RFP

Device was designed and assembled using Synbiota's Rapid DNA Prototyping (RDP) system. Part consists of a Lac promoter, medium RBS, and RFP open reading frame. In LacI- strains, or when cultured in the presence of IPTG, RFP expression is up-regulated. We created this device to use as a control in our experiments testing other devices.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 676
    Illegal AgeI site found at 788
  • 1000
    COMPATIBLE WITH RFC[1000]