Difference between revisions of "Part:BBa K1850012:Design"
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We selected <partinfo>BBa_I13453</partinfo>, an arabinose promoter, because it is a titratable promoter that allows for controlled expression of pili structural genes over a broad range of inducer concentrations. | We selected <partinfo>BBa_I13453</partinfo>, an arabinose promoter, because it is a titratable promoter that allows for controlled expression of pili structural genes over a broad range of inducer concentrations. | ||
| − | ''fim'' was amplified with the native ribosomal binding sites, so that we could maintain the dosing of the ' fim'' subunits. | + | ''fim'' was amplified with the native ribosomal binding sites, so that we could maintain the dosing of the ''fim'' subunits. |
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===Source=== | ===Source=== | ||
Revision as of 21:54, 18 September 2015
fim
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2585
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 868
Illegal AgeI site found at 899 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We selected BBa_I13453, an arabinose promoter, because it is a titratable promoter that allows for controlled expression of pili structural genes over a broad range of inducer concentrations.
fim was amplified with the native ribosomal binding sites, so that we could maintain the dosing of the fim subunits.
Source
The type I pili structural and transport genes from the fim operon were amplified from the E. coli K-12 genome.