Difference between revisions of "Part:BBa K1675002"

Revision as of 21:42, 18 September 2015

glsA, glutaminase1

GlsA encodes an acid-activated glutaminase, which is sufficient for an acid resistance system, and it is able to catalyze a reaction converting glutamate to glutamine and releasing the free gaseous ammonia. The free gaseous ammonia will consume the proton and increase the intracellular pH (Fig.1). The robust glutaminase activity only exists at pH 6.0 or lower. The highest activity is obtained at pH 4.0, followed by pH 5.0 and 6.0. In contrast, GlsA is not activated at pH 7.0 and 8.0. The dependence on pH makes it an effective tool to respond when necessary.

Fig.1 The mechanism of GlsA

Sequence and Features