Difference between revisions of "Part:BBa K1807002"
Line 2: Line 2:
 
<partinfo>BBa_K1807002 short</partinfo>
 
<partinfo>BBa_K1807002 short</partinfo>
  
−
This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from ''Escherichia coli''. PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the &#947;-phosphate of ATP to the end of the polyphosphate chain.
+
This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from ''Escherichia coli''. PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the &#947;-phosphate of ATP to the end of the polyphosphate chain (Akiyama et al., 1992). This enzyme is responsible for the formation of long chain polyphosphate molecules (up to a thousand orthophosphate residues long) - see illustration below.
 +
 
 +
<html>
 +
<body>
 +
<img src="https://static.igem.org/mediawiki/2015/d/d4/PPK_activity_York.jpeg" style="width:65%;height:auto">
 +
</body>
 +
</html>
 +
 
 +
Akiyama, Masahiro, E. Crooke, and Arthur Kornberg. "The polyphosphate kinase gene of Escherichia coli. Isolation and sequence of the ppk gene and membrane location of the protein." Journal of Biological Chemistry 267.31 (1992): 22556-22561.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:41, 18 September 2015

Escherichia coli Polyphosphate Kinase Enzyme

This part is a protein coding device containing the Polyphosphate Kinase (PPK) gene from Escherichia coli. PPK produces polyphosphate, more specifically PPK catalyzes the reversible conversion of the γ-phosphate of ATP to the end of the polyphosphate chain (Akiyama et al., 1992). This enzyme is responsible for the formation of long chain polyphosphate molecules (up to a thousand orthophosphate residues long) - see illustration below.

Akiyama, Masahiro, E. Crooke, and Arthur Kornberg. "The polyphosphate kinase gene of Escherichia coli. Isolation and sequence of the ppk gene and membrane location of the protein." Journal of Biological Chemistry 267.31 (1992): 22556-22561.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 396
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1750
  • 1000
    COMPATIBLE WITH RFC[1000]