Difference between revisions of "Part:BBa K1799020"

m
Line 2: Line 2:
 
<partinfo>BBa_K1799020 short</partinfo>
 
<partinfo>BBa_K1799020 short</partinfo>
  
−
Device was designed and assembled using Synbiota's GENtle software and Rapid DNA Prototyping (RDP) system.  Device consists of a strong promoter and ribosome binding site, followed by the LacI open reading frame. It is useful for generating the Lac repressor in chassis strains that do not produce it.
+
Device was designed and assembled using Synbiota's Rapid DNA Prototyping (RDP) system.  Device consists of a strong promoter and ribosome binding site, followed by the LacI open reading frame. It is useful for generating the Lac repressor in chassis strains that do not produce it.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:40, 18 September 2015

Constitutive generator of LacI

Device was designed and assembled using Synbiota's Rapid DNA Prototyping (RDP) system. Device consists of a strong promoter and ribosome binding site, followed by the LacI open reading frame. It is useful for generating the Lac repressor in chassis strains that do not produce it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 47
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]