Difference between revisions of "Part:BBa K1639000:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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This part contains additional BamHI restriction site between Toehold and GFP for replacing a new gene to downstream of Toehold sequence.
+
Important point in this part's design is that '''21 nucleotide linker''' and '''first 3 amino acids (9 nt)''' following it mustn't be changed. Because Toehold device and it's RBS region is optimaly engineered to work in optimum condition with this region. For practical use we added '''BamHI''' restriction site between GFP and these conserved sequence.
  
 
===Source===
 
===Source===

Latest revision as of 21:38, 18 September 2015


Toehold-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 772


Design Notes

Important point in this part's design is that 21 nucleotide linker and first 3 amino acids (9 nt) following it mustn't be changed. Because Toehold device and it's RBS region is optimaly engineered to work in optimum condition with this region. For practical use we added BamHI restriction site between GFP and these conserved sequence.

Source

Wyss Institute for Biologically Inspired Engineering. Sequence is taken from supplementary data of article: 'Toehold Switches: De-Novo-Designed Regulators of Gene Expression'. (PMID: 25417166)

References