Difference between revisions of "Part:BBa K1813065"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1813065 short</partinfo>
 
<partinfo>BBa_K1813065 short</partinfo>
  
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LacIR pelB AgCPR ptac pelB CYP6CM1
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With the <i> A. gambiae </i> NADPH p450 reductase (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</a></b></html>) regulated by the inducible LacI promoter and the CYP6M1vQ (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813004">BBa_K1813004</a></b></html>) enzyme under constitutive regulation, this part allows one to simultaneously gauge the effectiveness of the reductase and the CYP p450, localized to the bacterial periplasm, while remaining a viable method of expression control.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:36, 18 September 2015

LacIR pelB AgCPR ptac pelB CYP6CM1

With the A. gambiae NADPH p450 reductase ( BBa_K1813010) regulated by the inducible LacI promoter and the CYP6M1vQ ( BBa_K1813004) enzyme under constitutive regulation, this part allows one to simultaneously gauge the effectiveness of the reductase and the CYP p450, localized to the bacterial periplasm, while remaining a viable method of expression control.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BamHI site found at 1953
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1468
    Illegal NgoMIV site found at 3775
    Illegal AgeI site found at 1751
    Illegal AgeI site found at 2933
    Illegal AgeI site found at 3089
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3449