Difference between revisions of "Part:BBa K1813006"
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<h4>Part Function:</h4> | <h4>Part Function:</h4> | ||
| − | <p>The enzyme coded for by <i>nicC</i> converts 6-Hydroxynicitinoic acid (6-HNA) to 2,5-dihydroxypyridine (2,5-DHP).</p> | + | <p>The enzyme coded for by <i>nicC</i> is a 6-HNA monooxygenase that converts 6-Hydroxynicitinoic acid (6-HNA) to 2,5-dihydroxypyridine (2,5-DHP).</p> |
<h4>References:</h4> | <h4>References:</h4> | ||
[1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105 | [1] Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105 | ||
Revision as of 21:35, 18 September 2015
nicC
nicC Gen Bank accession no.: (Q88FY2.1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 244
- 1000COMPATIBLE WITH RFC[1000]
Background of nicC:
Part Description:
Codon optimized coding sequence of nicC, an enzyme from the Pseudmonas putida bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813020.
Part Origin:
Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 [1].
Part Function:
The enzyme coded for by nicC is a 6-HNA monooxygenase that converts 6-Hydroxynicitinoic acid (6-HNA) to 2,5-dihydroxypyridine (2,5-DHP).