Difference between revisions of "Part:BBa K1796012"

 
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__NOTOC__
 
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<partinfo>BBa_K1796012 short</partinfo>
 
<partinfo>BBa_K1796012 short</partinfo>
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<partinfo>BBa_K1796012 parameters</partinfo>
 
<partinfo>BBa_K1796012 parameters</partinfo>
 
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 +
 +
==Parameter of Protein==
 +
<p> Number of amino acids: 136 </p>
 +
<p> </p>
 +
<p> Molecular weight: 14908.1 </p>
 +
<p> </p>
 +
<p> Theoretical pI: 6.48 </p>
 +
<p> </p>
 +
<p> Amino acid composition:  </p>
 +
<p> </p>
 +
<p> Ala (A)  15      11.0% </p>
 +
<p> </p>
 +
<p> Arg (R)  9      6.6% </p>
 +
<p> </p>
 +
<p> Asn (N)  4      2.9% </p>
 +
<p> </p>
 +
<p> Asp (D)  3      2.2% </p>
 +
<p> </p>
 +
<p> Cys (C)  2      1.5% </p>
 +
<p> </p>
 +
<p> Gln (Q)  7      5.1% </p>
 +
<p> </p>
 +
<p> Glu (E)  13      9.6% </p>
 +
<p> </p>
 +
<p> Gly (G)  12      8.8% </p>
 +
<p> </p>
 +
<p> His (H)  4      2.9% </p>
 +
<p> </p>
 +
<p> Ile (I)  11      8.1% </p>
 +
<p> </p>
 +
<p> Leu (L)  10      7.4% </p>
 +
<p> </p>
 +
<p> Lys (K)  6      4.4% </p>
 +
<p> </p>
 +
<p> Met (M)  5      3.7% </p>
 +
<p> </p>
 +
<p> Phe (F)  7      5.1% </p>
 +
<p> </p>
 +
<p> Pro (P)  4      2.9% </p>
 +
<p> </p>
 +
<p> Ser (S)  8      5.9% </p>
 +
<p> </p>
 +
<p> Thr (T)  6      4.4% </p>
 +
<p> </p>
 +
<p> Trp (W)  1      0.7% </p>
 +
<p> </p>
 +
<p> Tyr (Y)  0      0.0% </p>
 +
<p> </p>
 +
<p> Val (V)  9      6.6% </p>
 +
<p> </p>
 +
<p> Pyl (O)  0      0.0% </p>
 +
<p> </p>
 +
<p> Sec (U)  0      0.0% </p>
 +
<p> </p>
 +
<p> (B)  0          0.0% </p>
 +
<p> </p>
 +
<p> (Z)  0          0.0% </p>
 +
<p> </p>
 +
<p> (X)  0          0.0% </p>
 +
<p> </p>
 +
<p> Total number of negatively charged residues (Asp + Glu): 16 </p>
 +
<p> Total number of positively charged residues (Arg + Lys): 15 </p>
 +
<p> </p>
 +
<p> Atomic composition:Carbon      C              655 </p>
 +
<p> Hydrogen    H        1057 </p>
 +
<p> Nitrogen    N          189 </p>
 +
<p> Oxygen      O          194 </p>
 +
<p> Sulfur      S            7 </p>
 +
<p> </p>
 +
<p> Formula: C655H1057N189O194S7Total number of atoms: 2102 </p>
 +
<p> </p>
 +
<p> Extinction coefficients:Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water. </p>
 +
<p> </p>
 +
<p> Ext. coefficient    5625 </p>
 +
<p> Abs 0.1% (=1 g/l)  0.377, assuming all pairs of Cys residues form cystines </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Ext. coefficient    5500 </p>
 +
<p> Abs 0.1% (=1 g/l)  0.369, assuming all Cys residues are reduced </p>
 +
<p> </p>
 +
<p> Estimated half-life:The N-terminal of the sequence considered is C (Cys). </p>
 +
<p> </p>
 +
<p> The estimated half-life is: 1.2 hours (mammalian reticulocytes, in vitro). </p>
 +
<p>                             >20 hours (yeast, in vivo). </p>
 +
<p>                             >10 hours (Escherichia coli, in vivo). </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Instability index:The instability index (II) is computed to be 46.59 </p>
 +
<p> This classifies the protein as unstable. </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> </p>
 +
<p> Aliphatic index: 90.44 </p>
 +
<p> </p>
 +
<p> Grand average of hydropathicity (GRAVY): -0.055 </p>

Revision as of 21:35, 18 September 2015

nifX promoted from Paenibacillus sp. WLY78

Function: encode carrier proteins involved in the delivery of FeMoco to the MoFe protein , participate in FeMoco assembly in a chaperone-like function.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 325
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 325
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 325
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 325
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 136

Molecular weight: 14908.1

Theoretical pI: 6.48

Amino acid composition: 

Ala (A) 15 11.0%

Arg (R) 9 6.6%

Asn (N) 4 2.9%

Asp (D) 3 2.2%

Cys (C) 2 1.5%

Gln (Q) 7 5.1%

Glu (E) 13 9.6%

Gly (G) 12 8.8%

His (H) 4 2.9%

Ile (I) 11 8.1%

Leu (L) 10 7.4%

Lys (K) 6 4.4%

Met (M) 5 3.7%

Phe (F) 7 5.1%

Pro (P) 4 2.9%

Ser (S) 8 5.9%

Thr (T) 6 4.4%

Trp (W) 1 0.7%

Tyr (Y) 0 0.0%

Val (V) 9 6.6%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 16

Total number of positively charged residues (Arg + Lys): 15

Atomic composition:Carbon C 655

Hydrogen H 1057

Nitrogen N 189

Oxygen O 194

Sulfur S 7

Formula: C655H1057N189O194S7Total number of atoms: 2102

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 5625

Abs 0.1% (=1 g/l) 0.377, assuming all pairs of Cys residues form cystines

Ext. coefficient 5500

Abs 0.1% (=1 g/l) 0.369, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is C (Cys).

The estimated half-life is: 1.2 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 46.59

This classifies the protein as unstable.

Aliphatic index: 90.44

Grand average of hydropathicity (GRAVY): -0.055