Difference between revisions of "Part:BBa K1813064"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1813064 short</partinfo>
 
<partinfo>BBa_K1813064 short</partinfo>
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With the <i> A. gambiae </i> NADPH p450 reductase (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</a></b></html>) regulated by the inducible LacI promoter and the CYP6G1 (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813004">BBa_K1813004</a></b></html>) enzyme under constitutive regulation, this part allows one to simultaneously gauge the effectiveness of the reductase and the CYP p450, while remaining a viable method of expression control.
  
 
LacIR pelB AgCPR ptac pelB-CYP6G1
 
LacIR pelB AgCPR ptac pelB-CYP6G1

Revision as of 21:31, 18 September 2015

LacIR pelB AgCPR ptac pelB-CYP6G1

With the A. gambiae NADPH p450 reductase ( BBa_K1813010) regulated by the inducible LacI promoter and the CYP6G1 ( BBa_K1813004) enzyme under constitutive regulation, this part allows one to simultaneously gauge the effectiveness of the reductase and the CYP p450, while remaining a viable method of expression control.

LacIR pelB AgCPR ptac pelB-CYP6G1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BamHI site found at 1953
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1468
    Illegal NgoMIV site found at 3775
    Illegal NgoMIV site found at 4721
    Illegal AgeI site found at 1751
    Illegal AgeI site found at 2933
    Illegal AgeI site found at 3089
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3449
    Illegal BsaI.rc site found at 4730