Difference between revisions of "Part:BBa K632003:Experience"
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We verified the regulatory system in our experiment, because we wanted to control the expression of the gene constructed behind the OmpC promoter by red light. | We verified the regulatory system in our experiment, because we wanted to control the expression of the gene constructed behind the OmpC promoter by red light. | ||
We tested the effect of light on the expression of genes by controlling the light condition of E.coli transformed with the genetic circuit of the regulator. | We tested the effect of light on the expression of genes by controlling the light condition of E.coli transformed with the genetic circuit of the regulator. | ||
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Fig 1: The genetic circuit of test system. | Fig 1: The genetic circuit of test system. | ||
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Two sample owning the same genetic circuit were set in our test experiment, the one in dark, the other in red light, and each sample had three biologic repeats. Besides we designed a simple and practical device to control the light condition of E.coli as the following picture shown. | Two sample owning the same genetic circuit were set in our test experiment, the one in dark, the other in red light, and each sample had three biologic repeats. Besides we designed a simple and practical device to control the light condition of E.coli as the following picture shown. | ||
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Fig 2:A red LED powered by battery is set at the bottom of a cup to provide red light | Fig 2:A red LED powered by battery is set at the bottom of a cup to provide red light | ||
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Fig 3:Two light conditions in our test device. | Fig 3:Two light conditions in our test device. | ||
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After some times gropes and tests, we gained related data and did correlation analysis as showing from the following table. As we can see from it, the presence of red light can inhibit the expression of target genes. (In the test, we used GFP as the target gene). | After some times gropes and tests, we gained related data and did correlation analysis as showing from the following table. As we can see from it, the presence of red light can inhibit the expression of target genes. (In the test, we used GFP as the target gene). | ||
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Fig 4. The effect of red light on the expression of genes | Fig 4. The effect of red light on the expression of genes | ||
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Revision as of 21:16, 18 September 2015
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