Difference between revisions of "Part:BBa K1850000:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Edited out standard assembly restriction enzyme cut sites through site-directed mutagenesis
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We selected a rhamnose-inducible promoter ([https://parts.igem.org/Part:BBa_K902065]) with a strong ribosome binding site (B0034), since this promoter is titratable and would allow for controlled expression of the fimH adhesin.
  
 
===Source===
 
===Source===

Revision as of 21:14, 18 September 2015


pRha - fimH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We selected a rhamnose-inducible promoter ([1]) with a strong ribosome binding site (B0034), since this promoter is titratable and would allow for controlled expression of the fimH adhesin.

Source

fimH comes from the E. coli K-12 genome, Rhamnose promoter comes from BBa_K902065

References