Difference between revisions of "Part:BBa K519010:Experience"
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<I>iGEM UFSCar-Brasil [http://2015.igem.org/Team:UFSCar-Brasil.html]</I>
 
<I>iGEM UFSCar-Brasil [http://2015.igem.org/Team:UFSCar-Brasil.html]</I>
 
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This SmtA was improved to eliminate an internal restriction site for PstI, the 98th base was changed '''G''' -> '''A'''. No residues were changed.
 
This SmtA was improved to eliminate an internal restriction site for PstI, the 98th base was changed '''G''' -> '''A'''. No residues were changed.
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[[File: Alignment between improved SmtA and BBa_K519010.jpg|450px|thumb|center|'''Figure 1:''' Improved SmtA (<partinfo>BBa_K1620007</partinfo>) compared to <partinfo>BBa_K519010</partinfo>; this new sequence is biobrick compatible due the removal of a PstI restriction site previously present in <partinfo>BBa_K519010</partinfo>.]]
 
[[File: Alignment between improved SmtA and BBa_K519010.jpg|450px|thumb|center|'''Figure 1:''' Improved SmtA (<partinfo>BBa_K1620007</partinfo>) compared to <partinfo>BBa_K519010</partinfo>; this new sequence is biobrick compatible due the removal of a PstI restriction site previously present in <partinfo>BBa_K519010</partinfo>.]]
 
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Revision as of 21:12, 18 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K519010

User Reviews

UNIQ4084e4dcc793723b-partinfo-00000000-QINU

No review score entered. iGEM Nagahama_2014

We found the sequence of this part has the PstⅠ site. So we mutated C→T at 96 (BBa_K1342003)

BBa_K1620007

iGEM UFSCar-Brasil [http://2015.igem.org/Team:UFSCar-Brasil.html]

This SmtA was improved to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed.

Figure 1: Improved SmtA (BBa_K1620007) compared to BBa_K519010; this new sequence is biobrick compatible due the removal of a PstI restriction site previously present in BBa_K519010.
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UNIQ4084e4dcc793723b-partinfo-00000006-QINU