Difference between revisions of "Part:BBa K519010:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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We found the sequence of this part has the PstⅠ site. So we mutated C→T at 96 | We found the sequence of this part has the PstⅠ site. So we mutated C→T at 96 | ||
([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1342003 BBa_K1342003]) | ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1342003 BBa_K1342003]) | ||
| + | |} | ||
| + | |||
| + | <partinfo>BBa_K1620007 AddReview number</partinfo> | ||
| + | <I>iGEM UFSCar-Brasil [http://2015.igem.org/Team:UFSCar-Brasil.html]</I> | ||
| + | |width='60%' valign='top'| | ||
| + | This SmtA was improved to eliminate an internal restriction site for PstI, the 98th base was changed '''G''' -> '''A'''. No residues were changed. | ||
| + | </p> | ||
| + | |||
| + | [[File: Alignment between improved SmtA and BBa_K519010.jpg|450px|thumb|center|'''Figure 1:''' Improved SmtA (<partinfo>BBa_K1620007</partinfo>) compared to <partinfo>BBa_K519010</partinfo>; this new sequence is biobrick compatible due the removal of a PstI restriction site previously present in <partinfo>BBa_K519010</partinfo>.]] | ||
|}; | |}; | ||
<!-- End of the user review template --> | <!-- End of the user review template --> | ||
<!-- DON'T DELETE --><partinfo>BBa_K519010 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K519010 EndReviews</partinfo> | ||
Revision as of 21:10, 18 September 2015
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K519010
User Reviews
UNIQce0ccbd548ddcb8e-partinfo-00000000-QINU
|
No review score entered. iGEM Nagahama_2014 |
We found the sequence of this part has the PstⅠ site. So we mutated C→T at 96 (BBa_K1342003) |
No review score entered. iGEM UFSCar-Brasil [http://2015.igem.org/Team:UFSCar-Brasil.html] |width='60%' valign='top'| This SmtA was improved to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed. </p>
Figure 1: Improved SmtA (BBa_K1620007) compared to BBa_K519010; this new sequence is biobrick compatible due the removal of a PstI restriction site previously present in BBa_K519010.
|}; UNIQce0ccbd548ddcb8e-partinfo-00000006-QINU