Difference between revisions of "Part:BBa K1688001"
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− | The | + | The enzymes responsible for producing the mono-rhamnolipids (biosurfactants) are called RhlA and RhlB which are encoded by the genes <i>rhlA</i> and <i>rhlB</i> respectively. The protein encoded by these two genes make up the subunits of rhamnosyltransferase I. The RhlA gene (BBa_K1688002) codes for a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail and the RhlB (BBa_K1688003) connects this tail to a rhamnose molecule making a mono-rhamnolipid. The biobrick BBa_K1688001 is a composite part of BBa_K1688002 and BBa_K1688003. |
Revision as of 21:08, 18 September 2015
Mono-rhamnolipid genes
The enzymes responsible for producing the mono-rhamnolipids (biosurfactants) are called RhlA and RhlB which are encoded by the genes rhlA and rhlB respectively. The protein encoded by these two genes make up the subunits of rhamnosyltransferase I. The RhlA gene (BBa_K1688002) codes for a 3-(hydroxyalkanoyloxy) alkanoic acid (HAA) fatty acid tail and the RhlB (BBa_K1688003) connects this tail to a rhamnose molecule making a mono-rhamnolipid. The biobrick BBa_K1688001 is a composite part of BBa_K1688002 and BBa_K1688003.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 87
Illegal BamHI site found at 647
Illegal XhoI site found at 823
Illegal XhoI site found at 2137 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1030
Illegal NgoMIV site found at 1751
Illegal NgoMIV site found at 1864 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 312
Illegal BsaI site found at 1380
Illegal BsaI.rc site found at 496