Difference between revisions of "Part:BBa K1763000"

Revision as of 21:07, 18 September 2015

Apis mellifera (honeybee) silk fibroin 3

This is the coding region for the honeybee silk protein #3. Previous literature has shown that it is possible to generate honeybee fibers by expressing this protein recombinantly in e. coli and spinning the protein into fibers in vitro It does not contain any of the regulatory elements necessary for protein expression.

Usage and Biology

Because this is just the coding region for the honeybee silk sequence, it is not meant to be expressed on its own. For an expressible honey bee silk biobrick part, please see BBa_K1763007 .

Characterization

Protein Expression

Here is the SDS gel of our expression of the honeybee silk protein. It was purified using [http://wolfson.huji.ac.il/purification/PDF/Protein_Expression_Extraction/NOVAGEN_BugBuster_protein_extraction.pdf this inclusion body protocol]

Fig. 1 Expected size of product is 40.0 kDA

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 11: / Line 11: @@
 ===Characterization===
 #Protein Expression
-*Here is the SDS gel of our first attempt at expression of the honeybee silk protein. The purified protein should be in the lane labeled "inclusion body." It was purified using [http://wolfson.huji.ac.il/purification/PDF/Protein_Expression_Extraction/NOVAGEN_BugBuster_protein_extraction.pdf this inclusion body protocol]
+*Here is the SDS gel of our expression of the honeybee silk protein. It was purified using [http://wolfson.huji.ac.il/purification/PDF/Protein_Expression_Extraction/NOVAGEN_BugBuster_protein_extraction.pdf this inclusion body protocol]
+[[File:UCLA Honeybee silk SDS PAGE9.18.jpg|none|thumb|500px|'''Fig. 1''' Expected size of product is 40.0 kDA]]
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