Difference between revisions of "Part:BBa K1796006"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1796006 short</partinfo> | <partinfo>BBa_K1796006 short</partinfo> | ||
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<partinfo>BBa_K1796006 parameters</partinfo> | <partinfo>BBa_K1796006 parameters</partinfo> | ||
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+ | |||
+ | ==Parametre of Protein== | ||
+ | <p> Number of amino acids: 294 </p> | ||
+ | <p> </p> | ||
+ | <p> Molecular weight: 32136.5 </p> | ||
+ | <p> </p> | ||
+ | <p> Theoretical pI: 4.79 </p> | ||
+ | <p> </p> | ||
+ | <p> Amino acid composition: </p> | ||
+ | <p> </p> | ||
+ | <p> Ala (A) 26 8.8% </p> | ||
+ | <p> </p> | ||
+ | <p> Arg (R) 13 4.4% </p> | ||
+ | <p> </p> | ||
+ | <p> Asn (N) 16 5.4% </p> | ||
+ | <p> </p> | ||
+ | <p> Asp (D) 14 4.8% </p> | ||
+ | <p> </p> | ||
+ | <p> Cys (C) 6 2.0% </p> | ||
+ | <p> </p> | ||
+ | <p> Gln (Q) 13 4.4% </p> | ||
+ | <p> </p> | ||
+ | <p> Glu (E) 29 9.9% </p> | ||
+ | <p> </p> | ||
+ | <p> Gly (G) 30 10.2% </p> | ||
+ | <p> </p> | ||
+ | <p> His (H) 5 1.7% </p> | ||
+ | <p> </p> | ||
+ | <p> Ile (I) 22 7.5% </p> | ||
+ | <p> </p> | ||
+ | <p> Leu (L) 27 9.2% </p> | ||
+ | <p> </p> | ||
+ | <p> Lys (K) 14 4.8% </p> | ||
+ | <p> </p> | ||
+ | <p> Met (M) 11 3.7% </p> | ||
+ | <p> </p> | ||
+ | <p> Phe (F) 9 3.1% </p> | ||
+ | <p> </p> | ||
+ | <p> Pro (P) 8 2.7% </p> | ||
+ | <p> </p> | ||
+ | <p> Ser (S) 9 3.1% </p> | ||
+ | <p> </p> | ||
+ | <p> Thr (T) 17 5.8% </p> | ||
+ | <p> </p> | ||
+ | <p> Trp (W) 0 0.0% </p> | ||
+ | <p> </p> | ||
+ | <p> Tyr (Y) 8 2.7% </p> | ||
+ | <p> </p> | ||
+ | <p> Val (V) 17 5.8% </p> | ||
+ | <p> </p> | ||
+ | <p> Pyl (O) 0 0.0% </p> | ||
+ | <p> </p> | ||
+ | <p> Sec (U) 0 0.0% </p> | ||
+ | <p> </p> | ||
+ | <p> (B) 0 0.0% </p> | ||
+ | <p> </p> | ||
+ | <p> (Z) 0 0.0% </p> | ||
+ | <p> </p> | ||
+ | <p> (X) 0 0.0% </p> | ||
+ | <p> </p> | ||
+ | <p> Total number of negatively charged residues (Asp + Glu): 43 </p> | ||
+ | <p> Total number of positively charged residues (Arg + Lys): 27 </p> | ||
+ | <p> </p> | ||
+ | <p> Atomic composition:Carbon C 1400 </p> | ||
+ | <p> Hydrogen H 2248 </p> | ||
+ | <p> Nitrogen N 386 </p> | ||
+ | <p> Oxygen O 444 </p> | ||
+ | <p> Sulfur S 17 </p> | ||
+ | <p> </p> | ||
+ | <p> Formula: C1400H2248N386O444S17Total number of atoms: 4495 </p> | ||
+ | <p> </p> | ||
+ | <p> Extinction coefficients:This protein does not contain any Trp residues. Experience shows that </p> | ||
+ | <p> this could result in more than 10% error in the computed extinction coefficient. </p> | ||
+ | <p> </p> | ||
+ | <p> Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. </p> | ||
+ | <p> </p> | ||
+ | <p> Ext. coefficient 12295 </p> | ||
+ | <p> Abs 0.1% (=1 g/l) 0.383, assuming all pairs of Cys residues form cystines </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> Ext. coefficient 11920 </p> | ||
+ | <p> Abs 0.1% (=1 g/l) 0.371, assuming all Cys residues are reduced </p> | ||
+ | <p> </p> | ||
+ | <p> Estimated half-life:The N-terminal of the sequence considered is H (His). </p> | ||
+ | <p> </p> | ||
+ | <p> The estimated half-life is: 3.5 hours (mammalian reticulocytes, in vitro). </p> | ||
+ | <p> 10 min (yeast, in vivo). </p> | ||
+ | <p> >10 hours (Escherichia coli, in vivo). </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> Instability index:The instability index (II) is computed to be 38.27 </p> | ||
+ | <p> This classifies the protein as stable. </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> </p> | ||
+ | <p> Aliphatic index: 90.61 </p> | ||
+ | <p> </p> | ||
+ | <p> Grand average of hydropathicity (GRAVY): -0.185 </p> |
Revision as of 20:47, 18 September 2015
Dinitrogenase reductase(nifH)
Dinitrogenase reductase, important in nitrogen fixation.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Parametre of Protein
Number of amino acids: 294
Molecular weight: 32136.5
Theoretical pI: 4.79
Amino acid composition:
Ala (A) 26 8.8%
Arg (R) 13 4.4%
Asn (N) 16 5.4%
Asp (D) 14 4.8%
Cys (C) 6 2.0%
Gln (Q) 13 4.4%
Glu (E) 29 9.9%
Gly (G) 30 10.2%
His (H) 5 1.7%
Ile (I) 22 7.5%
Leu (L) 27 9.2%
Lys (K) 14 4.8%
Met (M) 11 3.7%
Phe (F) 9 3.1%
Pro (P) 8 2.7%
Ser (S) 9 3.1%
Thr (T) 17 5.8%
Trp (W) 0 0.0%
Tyr (Y) 8 2.7%
Val (V) 17 5.8%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 27
Atomic composition:Carbon C 1400
Hydrogen H 2248
Nitrogen N 386
Oxygen O 444
Sulfur S 17
Formula: C1400H2248N386O444S17Total number of atoms: 4495
Extinction coefficients:This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 12295
Abs 0.1% (=1 g/l) 0.383, assuming all pairs of Cys residues form cystines
Ext. coefficient 11920
Abs 0.1% (=1 g/l) 0.371, assuming all Cys residues are reduced
Estimated half-life:The N-terminal of the sequence considered is H (His).
The estimated half-life is: 3.5 hours (mammalian reticulocytes, in vitro).
10 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:The instability index (II) is computed to be 38.27
This classifies the protein as stable.
Aliphatic index: 90.61
Grand average of hydropathicity (GRAVY): -0.185