Difference between revisions of "Part:BBa K1729002"
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This translational start sequence includes the Kozak and codon-optimized sequence for the mitochondrial localization signal of ''Saccharomyces cerevisiae'' mitochondrial ribosomal protein [http://www.yeastgenome.org/locus/S000005319/overview ''MRPS12'']. The cleavage site was predicted based on identification of an R-10 motif (RxFxxTxxxx), which is typical of precursors cleaved sequentially by the yeast mitochondrial processing peptidase and mitochondrial intermediate peptidase (Branda ''et al''.). | This translational start sequence includes the Kozak and codon-optimized sequence for the mitochondrial localization signal of ''Saccharomyces cerevisiae'' mitochondrial ribosomal protein [http://www.yeastgenome.org/locus/S000005319/overview ''MRPS12'']. The cleavage site was predicted based on identification of an R-10 motif (RxFxxTxxxx), which is typical of precursors cleaved sequentially by the yeast mitochondrial processing peptidase and mitochondrial intermediate peptidase (Branda ''et al''.). | ||
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| + | The part sequence corresponds to nucleotides 694840-694739 on chromosome XIV of the ''S. cerevisiae'' S288C reference genome. | ||
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| + | [[File:MLS Cap.jpg|900 px|]] | ||
<div class="center" style="width: auto; margin-left: auto; margin-right: auto;">Screen capture from SnapGene®</div> | <div class="center" style="width: auto; margin-left: auto; margin-right: auto;">Screen capture from SnapGene®</div> | ||
Revision as of 20:43, 18 September 2015
Yeast MRPS12 mitochondrial localization signal
This translational start sequence includes the Kozak and codon-optimized sequence for the mitochondrial localization signal of Saccharomyces cerevisiae mitochondrial ribosomal protein [http://www.yeastgenome.org/locus/S000005319/overview MRPS12]. The cleavage site was predicted based on identification of an R-10 motif (RxFxxTxxxx), which is typical of precursors cleaved sequentially by the yeast mitochondrial processing peptidase and mitochondrial intermediate peptidase (Branda et al.).
The part sequence corresponds to nucleotides 694840-694739 on chromosome XIV of the S. cerevisiae S288C reference genome.
References
Branda, Steven S et al. "Prediction and Identification of New Natural Substrates of the Yeast Mitochondrial Intermediate Peptidase." J Biol Chem 270 (1995): 27366-73.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]