Difference between revisions of "Part:BBa K1587003"
 
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<center>'''Figure 1: Crotonyl-CoA formation catalyzed by Crt'''</center>
 
<center>'''Figure 1: Crotonyl-CoA formation catalyzed by Crt'''</center>
  
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We used [https://parts.igem.org/Part:BBa_K1587003 BBa_K1587003] with other genes to produce butyrate in Escherichia coli (Figure 2).
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We used [https://parts.igem.org/Part:BBa_K1587003 BBa_K1587003] with other genes to produce butyrate in ''Escherichia coli'' (Figure 2).
  
 
<center>[[File:TLSE ButMetabo.jpg]]</center>
 
<center>[[File:TLSE ButMetabo.jpg]]</center>

Latest revision as of 20:40, 18 September 2015

3-hydroxybutyryl-CoA dehydratase

Coding region for the 3-hydroxybutyryl-CoA dehydratase (Crt), an enzyme involved in the butyrate biosynthesis pathway of Clostridium acetobutylicum. This substrate of this enzyme is 3-hydroxybutyryl-coA and the product is Crotonyl-CoA (Figure 1).

TLSE crt.jpg
Figure 1: Crotonyl-CoA formation catalyzed by Crt

We used BBa_K1587003 with other genes to produce butyrate in Escherichia coli (Figure 2).

TLSE ButMetabo.jpg
Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in E. coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]