Difference between revisions of "Part:BBa K1745001"

 
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<partinfo>BBa_K1745001 short</partinfo>
 
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pMC001 exhibits the three Kai proteins: KaiA, KaiB, and KaiC, which constitute a circadian oscillator. This biobrick can be used to drive circadian processes. Oscillations can be tracked through the phosphorylation states of KaiC.  
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pMC001 exhibits the three Kai proteins: KaiA, KaiB, and KaiC, which constitute a circadian oscillator. This biobrick can be used to drive circadian processes. Oscillations can be tracked through the phosphorylation states of KaiC. In vitro analysis of the Kai system found that the robustness of the oscillator was sensitive to KaiA:KaiC stoichiometry (Nakajima, 2010). In order to investigate this ratio, KaiA expression is driven under an L-rhamnose inducible promoter Part BBa_K914003 while KaiB and KaiA are driven under a constitutive promoter Part BBa_J23100.
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K1745001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1745001 SequenceAndFeatures</partinfo>
  

Revision as of 20:40, 18 September 2015

KaiABC Oscillator

pMC001 exhibits the three Kai proteins: KaiA, KaiB, and KaiC, which constitute a circadian oscillator. This biobrick can be used to drive circadian processes. Oscillations can be tracked through the phosphorylation states of KaiC. In vitro analysis of the Kai system found that the robustness of the oscillator was sensitive to KaiA:KaiC stoichiometry (Nakajima, 2010). In order to investigate this ratio, KaiA expression is driven under an L-rhamnose inducible promoter Part BBa_K914003 while KaiB and KaiA are driven under a constitutive promoter Part BBa_J23100.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3014
    Illegal NheI site found at 3037
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 410
    Illegal XhoI site found at 477
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2945
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 540