Difference between revisions of "Part:BBa K1587000"

Revision as of 20:35, 18 September 2015

ho1 with RBS

Strong RBS (BBa_B0030). Heme oxygenase-1 (Ho1) coding region (BBa_K566022).BBa_K566022 ho1, along with pcyA, converts heme into the chromophore phycocyanobillin (PCB) (Figure 1).
The integrity of our construct was checked by sequencing using VF2 primer.

Figure 1: pcyA catalysed formation of PCB from biliverdin (BV) IXα. (Ken et al. 2009)

Ho1 is used to catalyse the formation of biliverdin (BV) IXα via heme. It is usully used in association with PcyA enabeling the formation phycocyanobilin (PCB), a chromophore of the phytochrome family of photoreceptors, photosynthetic light-harvesting complexes required for circadian clock.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1587000 short</partinfo>
-Strong RBS (BBa_B0030). Heme oxygenase-1 (Ho1) coding region (BBa_K566022).
+Strong RBS (BBa_B0030). Heme oxygenase-1 (Ho1) coding region (BBa_K566022).[https://parts.igem.org/Part:BBa_K566022 BBa_K566022]
 ho1, along with pcyA, converts heme into the chromophore phycocyanobillin (PCB) (Figure 1).<br>
 The integrity of our construct was checked by sequencing using VF2 primer.