Difference between revisions of "Part:BBa K1729002"
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<partinfo>BBa_K1729002 short</partinfo>
 
<partinfo>BBa_K1729002 short</partinfo>
  
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This RC23 translational start sequence includes the Kozak and optimized coding sequence for the mitochondrial localization signal of ''Saccharomyces cerevisiae'' mitochondrial ribosomal protein MRPS12.
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This translational start sequence includes the Kozak and codon-optimized sequence for the mitochondrial localization signal of ''Saccharomyces cerevisiae'' mitochondrial ribosomal protein [http://www.yeastgenome.org/locus/S000005319/overview ''MRPS12'']. The cleavage site was predicted based on identification of an R-10 motif (RxFxxTxxxx), which is typical of precursors cleaved sequentially by the yeast mitochondrial processing peptidase and mitochondrial intermediate peptidase (Branda ''et al''.).
  
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[[File:MRPS12 MLS2.jpg|900 px|]]
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<div class="center" style="width: auto; margin-left: auto; margin-right: auto;">Screen capture from SnapGene&reg;</div>
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'''References'''
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Branda, Steven S ''et al''. "Prediction and Identification of New Natural Substrates of the Yeast Mitochondrial Intermediate Peptidase." J Biol Chem 270 (1995): 27366-73.
  
 
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Revision as of 20:29, 18 September 2015

Yeast MRPS12 mitochondrial localization signal

This translational start sequence includes the Kozak and codon-optimized sequence for the mitochondrial localization signal of Saccharomyces cerevisiae mitochondrial ribosomal protein [http://www.yeastgenome.org/locus/S000005319/overview MRPS12]. The cleavage site was predicted based on identification of an R-10 motif (RxFxxTxxxx), which is typical of precursors cleaved sequentially by the yeast mitochondrial processing peptidase and mitochondrial intermediate peptidase (Branda et al.).

File:MRPS12 MLS2.jpg

Screen capture from SnapGene®

References

Branda, Steven S et al. "Prediction and Identification of New Natural Substrates of the Yeast Mitochondrial Intermediate Peptidase." J Biol Chem 270 (1995): 27366-73.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]