Difference between revisions of "Part:BBa K1795023"

Latest revision as of 20:26, 18 September 2015

GalK Integrator Cassette

We created a modified backbone that will allow users to quickly and easily create parts that can be integrated onto the E. coli genome. This backbone is designed as follows (where H1 refers to the first region homologous to the E. coli genome, and H2 refers to the second homologous region)

In order to insert a part onto the genome, the user simply needs to put a part onto this backbone (between the prefix and suffix), and PCR up the region between (and including) H1 and H2.

Users can put their parts onto this backbone with their preferred method of assembly. This part exhibits resistance to chloramphenicol and kanamycin. The parts integrated with this cassette will contain kanamycin resistance, and will be integrated in the galK region of the E. coli genome.

Homologous regions modeled after those in Elowitz et al 2002.

This part has been successfully used to integrate fluorescent proteins onto the E. coli genome. Visual confirmation of integration of CFP at the galK locus is shown via confocal microscopy: [1]

Sequence and Features