Difference between revisions of "Part:BBa K1595024"
Line 2: | Line 2: | ||
<partinfo>BBa_K1595024 short</partinfo> | <partinfo>BBa_K1595024 short</partinfo> | ||
− | T7 Promoter + Ribosome binding site + MBP + TEV + EcnB + 6x Hist tag + terminator. Coding device for anti-microbial peptide derived from Mannheimia haemolytica EcnB with a | + | T7 Promoter + Ribosome binding site + MBP + TEV + EcnB + 6x Hist tag + terminator. Coding device for anti-microbial peptide derived from Mannheimia haemolytica EcnB with a stabilization maltose-binding protein and TEV cutsite. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Line 17: | Line 17: | ||
<!-- --> | <!-- --> | ||
− | + | <h3>Overview of Characterization of MBP+EcnB BioBrick</h3> | |
+ | As described on the <a href="http://2012.igem.org/Team:Cornell/testing/project/wetlab/2">2012 Cornell iGEM wiki</a> section, <i>S. oneidensis</i> MR-1 is capable of shuttling electrons through the Mtr pathway to reduce extracellular metals because of the negative free energy change associated with these redox reactions. Thus, to encourage <i>Shewanella</i> to transfer electrons to an electrode, we poise the potential of an electrode in a three electrode system, controlled by a potentiostat, so that electron transfer is energetically favorable to the organism. In short, a potentiostat works by setting the potential of a working electrode (WE) with respect to a Ag/AgCl reference electrode (RE) by injecting current through a counter electrode (CE). |
Revision as of 20:23, 18 September 2015
MBP with EcnB from Mannheimia haemolytica
T7 Promoter + Ribosome binding site + MBP + TEV + EcnB + 6x Hist tag + terminator. Coding device for anti-microbial peptide derived from Mannheimia haemolytica EcnB with a stabilization maltose-binding protein and TEV cutsite.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 440
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 138
Overview of Characterization of MBP+EcnB BioBrick
As described on the <a href="http://2012.igem.org/Team:Cornell/testing/project/wetlab/2">2012 Cornell iGEM wiki</a> section, S. oneidensis MR-1 is capable of shuttling electrons through the Mtr pathway to reduce extracellular metals because of the negative free energy change associated with these redox reactions. Thus, to encourage Shewanella to transfer electrons to an electrode, we poise the potential of an electrode in a three electrode system, controlled by a potentiostat, so that electron transfer is energetically favorable to the organism. In short, a potentiostat works by setting the potential of a working electrode (WE) with respect to a Ag/AgCl reference electrode (RE) by injecting current through a counter electrode (CE).