Difference between revisions of "Part:BBa K1795015"

Latest revision as of 20:16, 18 September 2015

CFP-LVA under promoter R0011

Cyan fluorescent protein with LVA fast degradation tag under the control of strong lacI regulated promoter R0011. This part has been visualized both via plasmid-based expression and genome integration expression. Confocal microscopy (60x oil immersion objective on a Nikon A1R confocal) shows strong fluorescent signal even for single copy number expression. See merged CFP/YFP image: [1] Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1795015 short</partinfo>
-Cyan fluorescent protein with LVA fast degradation tag under the control of strong lacI regulated promoter R0011. This part has been visualized both via plasmid-based expression and genome integration expression. Confocal microscopy (60x oil immersion objective on a Nikon A1R confocal) shows strong fluorescent signal even for single copy number expression.
+Cyan fluorescent protein with LVA fast degradation tag under the control of strong lacI regulated promoter R0011. This part has been visualized both via plasmid-based expression and genome integration expression. Confocal microscopy (60x oil immersion objective on a Nikon A1R confocal) shows strong fluorescent signal even for single copy number expression. See merged CFP/YFP image: [https://static.igem.org/mediawiki/2015/8/89/WM150914_r0011_no_saturation_replicate_1_picture_4.png]
-[https://static.igem.org/mediawiki/2015/8/89/WM150914_r0011_no_saturation_replicate_1_picture_4.png]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===