Difference between revisions of "Part:BBa K1813006"
Line 25: Line 25:
  
 
<h4>References:</h4>
 
<h4>References:</h4>
−
Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105
+
1) Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105

Revision as of 19:58, 18 September 2015

nicC

nicC Gen Bank accession no.: (Q88FY2.1).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 244
  • 1000
    COMPATIBLE WITH RFC[1000]

Background of nicC

Part Description:

Codon optimized coding sequence of nicC, an enzyme from the ''Pseudmonas putida'' bacterium KT2240, for E.Coli. This composite part was used to make our part BBa_K1813006.

Part Origin:

Originally cloned and characterized by Jimenez et al. from Pseudmonas putida KT2240 (1).

Part Function:

The enzyme coded for by ''nicC'' converts 6-Hydroxynicitinoic acid (6-HNA) to 2,5-dihydroxypyridine (2,5-DHP).

References:

1) Jose I. Jimenez, Angeles Canales, Jesus Jimenez-Barbero, et al. (2008)Deciphering the genetic determinants for aerobic nicotinic acid degradation: The nic cluster from Pseudomonas putida KT2440. PNAS 105(32): doi10.1073/pnas.0802273105