Difference between revisions of "Part:BBa K1820001"
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<partinfo>BBa_K1820001 short</partinfo> | <partinfo>BBa_K1820001 short</partinfo> | ||
+ | <p>A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31, containing two tandem transcription sites, and a downstream ribosomal binding site (RBS).</p> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | <p> Transcption form this promoter is induced after the phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.</p> | ||
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+ | ===Sequence and Features=== | ||
+ | <span class='h3bb'></span> | ||
<partinfo>BBa_K1820001 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1820001 SequenceAndFeatures</partinfo> | ||
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<partinfo>BBa_K1820001 parameters</partinfo> | <partinfo>BBa_K1820001 parameters</partinfo> | ||
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+ | ===References=== | ||
+ | <p>Djordjevic GM, Klaenhammer TR (1997) Bacteriophage-triggered defense systems: phage adaptation and design improvements. Appl Environ Microbiol 63:4370–4376.</p> |
Revision as of 19:50, 18 September 2015
P335-Φ31 inducible middle promoter + RBS
A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31, containing two tandem transcription sites, and a downstream ribosomal binding site (RBS).
Usage and Biology
Transcption form this promoter is induced after the phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 42
References
Djordjevic GM, Klaenhammer TR (1997) Bacteriophage-triggered defense systems: phage adaptation and design improvements. Appl Environ Microbiol 63:4370–4376.