Difference between revisions of "Part:BBa K1595030"
 
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<partinfo>BBa_K1595030 short</partinfo>
 
<partinfo>BBa_K1595030 short</partinfo>
  
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T7 promoter + Ribosome binding site + EDA + GSG + NdeI + cel5a + BamHI + 6xHist tag + Terminator. Coding device for KDPG aldolase (EDA), a peptide-stabilizing protein, with a Gly-Ser-Gly linker and protein cel5a.
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T7 promoter + Ribosome binding site + EDA + GSG + NdeI + cel5a + BamHI + 6xHist tag + Terminator. Coding device for KDPG aldolase (EDA), a peptide-stabilizing protein, with a Gly-Ser-Gly linker and detectable protein cel5a.
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 19:46, 18 September 2015

EDA linked to cel5a via GSG linker

T7 promoter + Ribosome binding site + EDA + GSG + NdeI + cel5a + BamHI + 6xHist tag + Terminator. Coding device for KDPG aldolase (EDA), a peptide-stabilizing protein, with a Gly-Ser-Gly linker and detectable protein cel5a.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1965
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 641
    Illegal NgoMIV site found at 1038
    Illegal NgoMIV site found at 1494
    Illegal AgeI site found at 1758
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1755