Difference between revisions of "Part:BBa K1595030"
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<partinfo>BBa_K1595030 short</partinfo> | <partinfo>BBa_K1595030 short</partinfo> | ||
− | Coding device for KDPG aldolase (EDA), a peptide-stabilizing protein, with a Gly-Ser-Gly linker and protein cel5a. | + | T7 promoter + Ribosome binding site + EDA + GSG + NdeI + cel5a + BamHI + 6xHist tag + Terminator. Coding device for KDPG aldolase (EDA), a peptide-stabilizing protein, with a Gly-Ser-Gly linker and protein cel5a. |
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Revision as of 19:46, 18 September 2015
EDA linked to cel5a via GSG linker
T7 promoter + Ribosome binding site + EDA + GSG + NdeI + cel5a + BamHI + 6xHist tag + Terminator. Coding device for KDPG aldolase (EDA), a peptide-stabilizing protein, with a Gly-Ser-Gly linker and protein cel5a.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1965
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 641
Illegal NgoMIV site found at 1038
Illegal NgoMIV site found at 1494
Illegal AgeI site found at 1758 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1755