Difference between revisions of "Part:BBa K1820003"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1820003 short</partinfo>
 
<partinfo>BBa_K1820003 short</partinfo>
 +
<p>A divergent phage-inducible promoter from the genetic switch region of the P335 species lactococcal phage Φ31 (BBa_K1820002), with the native ribosomal binding site (RBS)</p>
  
−
Add
 
−
 
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
<p> This promoter region contains a leftward and rightward promoter. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes, and translation beginning at the RBS.  </p>
 +
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
 
 +
===Sequence and Features===
 +
<span class='h3bb'></span>
 
<partinfo>BBa_K1820003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1820003 SequenceAndFeatures</partinfo>
  
Line 17: Line 19:
 
<partinfo>BBa_K1820003 parameters</partinfo>
 
<partinfo>BBa_K1820003 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
===References===
 +
<p>Durmaz E, Madsen SM, Israelsen H, Klaenhammer TR (2002) Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor. J Bacteriol 184:6532–6544.</p>

Revision as of 19:45, 18 September 2015

P335-Φ31phage genetic switch-divergent promoter + native RBS

A divergent phage-inducible promoter from the genetic switch region of the P335 species lactococcal phage Φ31 (BBa_K1820002), with the native ribosomal binding site (RBS)

Usage and Biology

This promoter region contains a leftward and rightward promoter. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes, and translation beginning at the RBS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Durmaz E, Madsen SM, Israelsen H, Klaenhammer TR (2002) Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor. J Bacteriol 184:6532–6544.