Difference between revisions of "Part:BBa K1633003"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1633003 short</partinfo>
 
<partinfo>BBa_K1633003 short</partinfo>
Line 12: Line 11:
 
<partinfo>BBa_K1633003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1633003 SequenceAndFeatures</partinfo>
  
 +
 +
==== Usage and Biology ====
 +
 +
This part is a shRNA designed to target and degrade MOR mRNA. When this shRNA sequence is cut by restriction enzyme and then integrated into mammalian vector, this shRNA can play a RNAi function in mammalian cell lines. When the shRNA vector of MOR is transfected into mammalian cells, the shRNA hairpin structure is cleaved by Dicer into siRNA of MOR and loaded into the RISC. The siRNA-RISC complex targets at MOR mRNA under the guide of siRNA sequence and cleave the MOR mRNA.
 +
 +
==== CHARACTERIZATION ====
 +
 +
Interference efficiency of MOR siRNA-1 plasmid
 +
 +
To ensure the interference efficiency, MOR siRNA-1 plasmid was,transfected into the mouse neuroblastoma cell line Neuro2A. Efficient knockdown of MOR by MOR siRNA-1 in Neuro2A cells is observed.
 +
 +
[[File:NJU-China-parts-fig 2.png|400px]]
 +
 +
Figure 2. Relative level of MOR mRNA in Neuro2A cell after transfection of MOR siRNA-1 plasmid.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 19:29, 18 September 2015

MOR siRNA-1 (siRNA for mouse Mu opioid receptor)

This part is a short hairpin RNA (shRNA) sequence. When this shRNA sequence is cut by restriction enzyme and then integrated into pcDNA 6.2 vector, this shRNA can play a RNAi function in mammalian cell lines such as HEK293 cell. When the shRNA vector of MOR is transfected into HEK293 cells, the shRNA hairpin structure is cleaved by Dicer into siRNA of MOR and loaded into the RISC. The siRNA-RISC complex targets at Mus musculus MOR mRNA under the guide of siRNA sequence and cleave the MOR mRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This part is a shRNA designed to target and degrade MOR mRNA. When this shRNA sequence is cut by restriction enzyme and then integrated into mammalian vector, this shRNA can play a RNAi function in mammalian cell lines. When the shRNA vector of MOR is transfected into mammalian cells, the shRNA hairpin structure is cleaved by Dicer into siRNA of MOR and loaded into the RISC. The siRNA-RISC complex targets at MOR mRNA under the guide of siRNA sequence and cleave the MOR mRNA.

CHARACTERIZATION

Interference efficiency of MOR siRNA-1 plasmid

To ensure the interference efficiency, MOR siRNA-1 plasmid was,transfected into the mouse neuroblastoma cell line Neuro2A. Efficient knockdown of MOR by MOR siRNA-1 in Neuro2A cells is observed.

NJU-China-parts-fig 2.png

Figure 2. Relative level of MOR mRNA in Neuro2A cell after transfection of MOR siRNA-1 plasmid.