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===Sequence and Features===
 
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===Functional Parameters===
 
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Revision as of 19:28, 18 September 2015

P335-Φ31 inducible middle promoter

A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31, containing two tandem transcription sites.

Usage and Biology

Transcption form this promoter is induced after phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42


References

Djordjevic GM, Klaenhammer TR (1997) Bacteriophage-triggered defense systems: phage adaptation and design improvements. Appl Environ Microbiol 63:4370–4376.

Durmaz E, Madsen SM, Israelsen H, Klaenhammer TR (2002) Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor. J Bacteriol 184:6532–6544.