Difference between revisions of "Part:BBa K1820000"
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<partinfo>BBa_K1820000 short</partinfo>
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<p>A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31, containing two tandem transcription sites.</p>  
  
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A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage phi-31, containing two tandem transcription sites.
 
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===Usage and Biology===
 
===Usage and Biology===
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<p> Transcption form this promoter is induced after phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.</p>
  
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1820000 SequenceAndFeatures</partinfo>
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===Sequence and Features===
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<partinfo>BBa_K1820014 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1820000 parameters</partinfo>
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<partinfo>BBa_K1820014 parameters</partinfo>
 
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===References===
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<p>Djordjevic GM, Klaenhammer TR (1997) Bacteriophage-triggered defense systems: phage adaptation and design improvements. Appl Environ Microbiol 63:4370–4376.</p>
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<p>Durmaz E, Madsen SM, Israelsen H, Klaenhammer TR (2002) Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor. J Bacteriol 184:6532–6544.</p>

Revision as of 19:26, 18 September 2015

CP8_RBS_sfGFP(Bs)_Terminator

A phage-inducible promoter natively located in the middle of the genome of the P335 species lactococcal phage Φ31, containing two tandem transcription sites.

Usage and Biology

Transcption form this promoter is induced after phage genome is inserted into the bacterial cell, resulting in downstream expression in the initial stages of infection. It has been previously demonstrated that expression is successfully induced from this promoter and only occurs during phage infection.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 99


References

Djordjevic GM, Klaenhammer TR (1997) Bacteriophage-triggered defense systems: phage adaptation and design improvements. Appl Environ Microbiol 63:4370–4376.

Durmaz E, Madsen SM, Israelsen H, Klaenhammer TR (2002) Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor. J Bacteriol 184:6532–6544.