Difference between revisions of "Part:BBa K1587006"
 
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Uses a positively regulated promoter (pompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by cph8 red light receptor (cph1-envZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes.
 
Uses a positively regulated promoter (pompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by cph8 red light receptor (cph1-envZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes.
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Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfortunatly we did not succeed in cloning the final construct with ho1 and pcyA (Figure 1).<br>
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Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfortunatly we did not succeed in cloning the final construct with the cyrcadian switch genes.<br>
 
The integrity of our construct was checked by sequencing using VF2 primer and cI specific primers.
 
The integrity of our construct was checked by sequencing using VF2 primer and cI specific primers.
  

Latest revision as of 19:19, 18 September 2015

pompC and lacI box regulated cI expression

Uses a positively regulated promoter (pompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by cph8 red light receptor (cph1-envZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes. Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfortunatly we did not succeed in cloning the final construct with the cyrcadian switch genes.
The integrity of our construct was checked by sequencing using VF2 primer and cI specific primers.


TLSE cI.jpg
Figure 1: Genetic construct for pompC and lacI box regulated cI expression

The aim of this construct was to create a circadian switch to alternate the expression of two sets of genes (Figure 2). This enables the production of one protein by night and another by day.

TLSE-Circadian.JPG
Figure 2: Circadian gene expression switch

This regulation system is a NOT logic gate controled by a day/night.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]