Difference between revisions of "Part:BBa K1813010"
 
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<h2> Background </h2>
 
<h2> Background </h2>
  
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Transferring electrons from NADPH to a cytochrome p450 enzyme, this enzyme replenishes electrons used by a cytochrome p450 in the catalysis of a reaction ensuring the reusability of the enzyme.
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Transferring electrons from NADPH to a cytochrome p450 enzyme, this enzyme replenishes electrons used by a cytochrome p450 in the catalysis of a reaction ensuring the reusability of the enzyme [1].
 
This coding sequence was synthesized and is codon optimized for <i> E. coli</i>. It was originally identified in <i> Anopheles gambiae</i>, the Anopheles Mosquito [2].
 
This coding sequence was synthesized and is codon optimized for <i> E. coli</i>. It was originally identified in <i> Anopheles gambiae</i>, the Anopheles Mosquito [2].
 
This part is also used in<html><b> <a href="https://parts.igem.org/Part:BBa_K1813018"> BBa_K1813018</a></b></html>.
 
This part is also used in<html><b> <a href="https://parts.igem.org/Part:BBa_K1813018"> BBa_K1813018</a></b></html>.
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<h2> References </h2>
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[1] Karunker, I., Morou, E., Nikou, D., Nauen, R., Sertchook, R., Stevenson, B. J., ... & Vontas, J. (2009). Structural model and functional characterization of the Bemisia tabaci CYP6CM1vQ, a cytochrome P450 associated with high levels of imidacloprid resistance. Insect biochemistry and molecular biology, 39(10), 697-706.

Latest revision as of 19:18, 18 September 2015

A. gamb NADPH Reductase

The Anopheles gambiae NADPH - p450 reductase is a redox enzyme critical to the reuse of cytochrome p450 enzymes dependent on a supply of electrons for function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 473
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 271
    Illegal AgeI site found at 1453
    Illegal AgeI site found at 1609
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1969


Background

Transferring electrons from NADPH to a cytochrome p450 enzyme, this enzyme replenishes electrons used by a cytochrome p450 in the catalysis of a reaction ensuring the reusability of the enzyme [1]. This coding sequence was synthesized and is codon optimized for E. coli. It was originally identified in Anopheles gambiae, the Anopheles Mosquito [2]. This part is also used in BBa_K1813018.


References

[1] Karunker, I., Morou, E., Nikou, D., Nauen, R., Sertchook, R., Stevenson, B. J., ... & Vontas, J. (2009). Structural model and functional characterization of the Bemisia tabaci CYP6CM1vQ, a cytochrome P450 associated with high levels of imidacloprid resistance. Insect biochemistry and molecular biology, 39(10), 697-706.