Difference between revisions of "Part:BBa K1758314"
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===Functional Parameters===
 
===Functional Parameters===
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Revision as of 19:17, 18 September 2015

Chromium responsive promoter under T7-promoter with UTR-sfGFP

Chromium induceble promoter under the control of a T7 promoter with 5´untranslated region and sfGFP for detection

Usage and Biology

This part is essential for our in vitro characterization of our chromium sensor. The edition of T7 promoter to BBa_K1758313 enables us to characterize tihs sensor in our CFPS.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 167
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 260