Difference between revisions of "Part:BBa K1679013:Design"

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===Design Notes===
 
===Design Notes===
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We use a special RBS to make the device temperature-sensitive. After adding a promoter，the secondary structure of the RBS after transform would be folded when the temperature is below 42°C, so that the ribosome can't translated RFP. When the temperature rises above 42°C, the secondary structure of the RBS after transform would be unfolded and produce RFP.
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We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 42°C, the secondary structure of the RBS after transform would be unfolded and produce RFP.  
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We designed this part for measurement of the switch activity of K115001.
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===Source===
 
===Source===
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===References===
 
===References===
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Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.

Revision as of 19:13, 18 September 2015

RNA thermometer (ROSE)+mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 657
    Illegal AgeI site found at 769
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use a RNA thermometer to make the device temperature-sensitive. When the temperature is below 37°C,the secondary structure of the RBS after transform would be folded, so that the ribosome can't translated RFP. When the temperature rises above 42°C, the secondary structure of the RBS after transform would be unfolded and produce RFP. We designed this part for measurement of the switch activity of K115001.

Source

BBa_K115001 BBa_E1010

References

Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches[J]. Nature Reviews Microbiology, 2012, 10(4):255-265.